Monroig O , Navarro JC , Amat F & Hontoria F ( 2007 ) Enrichment
نویسندگان
چکیده
Several types of liposomes were used to enrich Artemia nauplii in vitamin A, vitamin C and free methionine. In a first experiment, unilamellar liposomes formulated with krill phospholipid extract and retinyl palmitate demonstrated their capability to enhance the retinol content of Artemia nauplii. Furthermore, the increase in retinol was related to the amount of retinyl palmitate included in the liposomes as vitamin A source. These findings yield the possibility of using such vesicles to bioencapsulate simultaneously both vitamin A and essential fatty acids present in the krill phospholipid extract. A second enrichment was carried out with unilamellar liposomes composed of soybean phosphatidylcholine and loaded with sodium ascorbate as vitamin C source. Our results did not show that vitamin C content in the nauplii could be increased using unilamellar liposomes. This was most likely due to the degradation of the vitamin C during enrichment as well as the ascorbate leakage. Finally, a third experiment assessed enrichment in free methionine using liposomes of different lamellarity (unilamellar or multilamellar) and composed of either soybean phosphatidylcholine or dipalmitoyl phosphatidylcholine, both combined with cholesterol as a membrane stabilizer. Results indicated that multilamellar liposomes represent a useful tool to deliver methionine to Artemia nauplii. Enhanced protection given by their multiple bilayers in comparison to unilamellar liposomes could account for the higher ability displayed by multilamellar vesicles for free methionine bioencapsulation.
منابع مشابه
Studies on the characterisation of biomarkers of nutritionally-derived stress in paralarval cultures of the common octopus (Octopus vulgaris).
1 Instituto de Acuicultura Torre de la Sal. (IATS-CSIC), 12595 Ribera de Cabanes, Spain 2 Instituto Español de Oceanografía, Centro Oceanográfico de Vigo, Apartado 1552, 36200 Vigo, Spain 3 Instituto Español de Oceanografía, Centro Oceanográfico de Canarias, Avd. Tres de Mayo 73, 38005 Santa Cruz de Tenerife, Spain 4 Departamento de Zoología, Universidad de Granada, Campus de Fuentenueva, 18071...
متن کاملBiosynthesis of essential fatty acids in Octopus vulgaris (Cuvier, 1797): Molecular cloning, functional characterisation and tissue distribution of a fatty acyl elongase Authors
متن کامل
Elsevier Editorial System(tm) for Aquaculture Title: Oxidative Stability and Changes in the Particle Size of Liposomes Used in the Artemia Enrichment
The oxidative stability and the particle size of several types of liposomes were assessed in order to characterize their behaviour when submitted to the aggressive conditions of the Artemia enrichments. Results show that all liposomes tested in this study were much more oxidatively stable than a commercial product based on fish oil emulsion. Whereas the initial thiobarbituric acid reactive subs...
متن کاملEffects of Hypoxia, and the Balance between Enrichment, on Coastal Fishes and Fisheries Hypoxia and
A reduct ion in dissolved oxygen concentrat ion is one of the most important direct effects of nutrient over-enrichment of coastal waters on fishes. Because hypoxia can cause nlortality~ reduced growth rates, and altered distributions and behaviors o f fishes, as wel l as changes in the relative importance o f organisms andpathways of carbon f low within f o o d webs, hypoxia and anoxia can lea...
متن کاملMolecular cloning and functional characterization of a putative Elovl4 gene and its expression in response to dietary fatty acid profiles in orange-spotted grouper Epinephelus coioides. Aquac
This is the peer reviewed version of the following article: Li, S., Monroig, Ó., Navarro, J. C., Yuan, Y., Xu, W., Mai, K., Tocher, D. R. and Ai, Q. (2017), Molecular cloning and functional characterization of a putative Elovl4 gene and its expression in response to dietary fatty acid profiles in orange-spotted grouper Epinephelus coioides. Aquac Res, 48: 537–552, which has been published in fi...
متن کامل